Researchers from MMM are working to improve how we find out what bacteria are causing infections in joints.
MMM Researchers are showing that you can take the joint fluid, break open all the bacteria, and extract their DNA. (Bacteria have DNA too). This bacterial DNA can be analysed in hours, giving faster results, with the potential to ensure patients are given the right treatment sooner. The technique could also be used to diagnose other infections in the future.
Currently the work is still in development, but an early report can be found here
We are also testing the new Oxford Nanopore Technologies ‘Voltrax’ sample preparation device:
— Nicholas Sanderson (@nickdsanderson) November 29, 2017
Today @tree_street @nanopore sequenced a PJI sample using a voltrax for library prep (~45mins). We detected the infection species within 12 mins of sequencing using CRuMPIT (https://t.co/kfyYL2wIB5), exciting times. @ModMedMicro pic.twitter.com/0IFuDmoaaj
— Nicholas Sanderson (@nickdsanderson) December 13, 2017
— Nicholas Sanderson (@nickdsanderson) November 17, 2017
The computer program used to analyse the DNA has been provisionally called CRuMPIT: Clinical Real-time Metagenomic Pathogen Identification Test, by the bioinformatician, Nick Sanderson. Whilst this might seem unusual, it follows a recent trend for scientists using bakery-related names for genomic analysis tools: like PPANINI, ShortBRED, strudel and galaxy_picrust. We are happy to add some UK specialities to the mix!
More about our work on joint infections can be found here: